FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_sige45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_sige45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7007387
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCTCAGACCTATCAACCTGATCATCTTT166030.23693567944798824No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCC134670.19218290641004984No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGGGTTTCCCCATTCGGAAAT114540.16345607856394972No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGAGACT109570.1563635631940979No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTC106300.15169705911775672No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAG102500.14627421034402696No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAA97990.13983814508888975No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACT95400.13614204552995288No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCG91880.131118775086919No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGAC90460.1290923421241042No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCG86550.12351251614902958No Hit
CGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAG83590.11928840236738744No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAA82230.11734759333257888No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA77410.1104691377827427No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCT76570.10927040279065507No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGAC76400.109027801661304No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTCGGTGGAGGGGATTCTCACCCCTCTT74850.10681585018780895No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTA72420.10334808110355542No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGC71050.10139300141407917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGT31200.042.683799
TCGATCG32100.041.8235133
CGATCGA32800.041.664514
TTAGTAT31900.041.6366211
CGATTAG32700.040.8327838
ATTAGTA33200.040.11218610
AGTATCT34700.038.97228213
GATCGAT35450.038.254095
CCATGTG37050.036.30972727
GTATCTG37150.036.12351614
TATCTGT38550.035.16934615
CATGTGT38600.034.7620828
GTGTCGC36550.033.88224831
TCCATGT40000.033.6316426
GTCGCCA37150.033.05787333
TGTCGCC37350.032.97274832
CTCCATG41300.032.57394825
CACACTT42600.031.25801838
GCGCACG12750.031.132
ACACTTC42900.031.12073139
Produced by FastQC (version 0.11.2)