FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_sige35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_sige35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5887241
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG327830.5568482757882682No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA317090.5386054350416434No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC228150.38753297172648443No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAG193130.32804840161970605No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAGCGCGGCGCAATTCA156010.2649967956127497No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTT146040.2480618680295235No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA144690.2457687735222662No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGATTAA143550.24383238260502668No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGA127830.2171305710094083No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACT119620.2031851592282361No Hit
CGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAAC118700.20162245778625335No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTT116920.19859897021372153No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAGCGCGGCGCAATTCAA115720.19656066398504835No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTT107110.18193581679431842No Hit
GGCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGA105300.17886137156606974No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTA100590.1708610196185276No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAAC95410.16206233106475512No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCT88810.15085164680705274No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAATGAAAAAAGCTTATAAGAAACCTCGC83100.14115270633561627No Hit
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAGT81880.13908042833646525No Hit
GTTTTGGTTAAAACTGTTAGTTTTGCCAGTTATATTTAAGTGCGTGTTTACGAGATCGCCTCTCGGCTCGCAGCT80900.13741581158304883No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGCAGCTGGCGGCCATTAATCTCATAT79960.13581913837058818No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAAC75130.12761495580017873No Hit
GTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAGTC73170.1242857222933459No Hit
GAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGAT72800.12365724453950501No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGG70150.11915598495118511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGA34600.035.021191
TAGGGGG11250.032.5055850
TACGGAT36450.031.0653461
ACGGATT85050.029.1440581
GATTCGA93900.027.4150184
CGGATTC95550.027.0536272
TACAAGG118900.026.5507648
ATTCGAC99250.025.8298535
GGATTCG103650.025.0376423
TTACAAG127900.024.7903587
GTGATAG51050.024.672464
TCGACAG105000.024.5124867
AGGTAGC19200.023.9006866
ACAAGGA133650.023.6459589
CGTGATA53600.023.5646573
TTAGGGG8650.023.53080649
GCGTGAT54900.023.5111372
AGGAAGG136000.023.41415412
GAGGGAA136100.023.39675118
TTCGACA110800.023.3862256
Produced by FastQC (version 0.11.2)