FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_s877_sigg45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_s877_sigg45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7783787
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGT16050.025.3654869
TTAGTAT17250.023.99940711
CGATTAG19600.020.9477928
ATTAGTA20250.020.61509910
TAGGGGG17300.020.14099150
GACTAGT1108.22543E-418.8240053
CGATCGA29350.016.9308974
TCGATCG28900.016.598563
ACTAACC15850.016.54228256
AGTATCT25850.016.28293613
CACCTAG4750.015.981535
TCTCGAG22800.015.73655356
TTAGGGG16100.015.64176649
TAGGTCC17050.015.58035947
GCTCTAG23050.014.96622634
GAGTCGA19600.014.9654034
GTGATAG20600.014.9099523
ACCTAGT6100.014.7067286
CTAGCAG22900.014.61270637
GGTCCCG19250.014.15745449
Produced by FastQC (version 0.11.2)