FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_s87745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_s87745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8429084
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGT17550.030.4707859
TTAGTAT20800.026.04161611
ATTAGTA21000.025.79329710
CGATTAG23250.023.4459788
AGTATCT28800.019.0468613
GCTAGCA9350.018.08030767
CCTAACT9450.017.164832
TCGATCG36500.016.9242863
CTAGCAA9650.016.80349568
GTGTCGC31300.016.09092131
GACCTAT32850.015.96298453
CGCTAGC9150.015.83614766
GTATCTG34500.015.79989414
GATCGAT39000.015.3060145
GACTAGT2503.6675374E-715.1834124
TAGTATC36450.015.14384212
GCCACAC39650.014.70377236
GGGGGTA9450.014.60333765
GCCTAAC12750.014.3506171
CCTCGAT44600.014.2425541
Produced by FastQC (version 0.11.2)