FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_csfg_sige35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_csfg_sige35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6782548
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG247760.3652904483683713No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA206020.30375015407189154No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC150500.22189301129899855No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAG128550.18953054220921106No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA100510.14818914661569665No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAGCGCGGCGCAATTCA99000.14596284464186615No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGATTAA94380.13915124522524575No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGA93690.13813392842925695No Hit
GGCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGA91190.13444799800900784No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTT82660.12187160341511774No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACT75020.11060740005083636No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTT74400.10969328930661457No Hit
CGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAAC73820.10883815344911676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGA28150.028.6966571
TAGGGGG11000.027.91338350
CTAGCAA8600.024.47197268
ATTCGAC67800.023.8676435
GTGATAG35150.023.658664
GATTCGA68400.023.6095754
TCGACAG70350.023.0008077
CGCTAGC6950.022.3390866
ACGGATT69900.022.323131
GCTAGCA9300.022.25884867
TACAAGG95000.021.8620688
CCCACGT29250.021.4802531
TGATAGC39900.021.2729935
CGACAGG76700.021.1857538
TAGCCTC38800.021.0734188
GGATTCG78850.020.7012393
GACAGGG79250.020.4165489
TTACAAG103050.020.3224037
CGGATTC83300.020.0531252
ACAGGGA81850.019.89430810
Produced by FastQC (version 0.11.2)