FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_csfg35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_csfg35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8297743
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA229120.27612327834207445No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG155510.18741240841033519No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAG122470.14759435186170505No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC113080.13627802162588068No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA102820.12391321350878186No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTT91130.10982504519602498No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACT88360.1064867880338063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG14600.026.46474850
ATTCGAC79150.022.5376015
GATTCGA79800.022.4418544
CCCACGT29100.020.640151
TCGACAG86700.020.6136637
TACAAGG76050.020.2334378
GGATTCG88100.020.2110543
GCGAGGT110950.019.9739671
GAGGGAA78000.019.90381218
CGACAGG89300.019.8584658
ACTAGAT82750.019.51116252
CGGATTC96250.019.4693262
TACTAGA83450.019.34737851
TTCGACA94450.019.0689226
GTGATAG31250.018.9920864
TGCTATA86350.018.61905365
GTACTAG83550.018.53969250
AGGGGGG33300.018.33714351
GTGATCT32550.018.33692666
ACGGATT93600.018.2920531
Produced by FastQC (version 0.11.2)