FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_79t45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_79t45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7048888
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAT32550.015.579926552
TAGGGGG9450.014.60249950
TACTAGA34150.014.54691251
CGGGGAG17650.014.08239651
TAGAGTA36550.013.96945746
CTAGATG36600.013.76156253
GTACTAG37150.013.27933350
GCGAGGT57300.012.7723271
GTGATAG20300.012.7504213
GAGTACT38700.012.56927648
AGAGTAC39500.012.48929647
TGCTATA36900.012.34185865
AGTACTA41400.012.16593349
ATGCTAT38100.011.953052564
AATGCTA41150.011.56990763
ATAGAGT44350.011.51259745
GGGGGGA29300.011.18604720
TTAGGGG9050.011.05458149
AGGTAGC16550.011.050186
GGTAGCG15800.010.9188457
Produced by FastQC (version 0.11.2)