FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_79t35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_79t35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7788090
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA212990.27348168806472445No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG190590.24471982219003632No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC133510.1714284246843578No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAG106870.13722234848338938No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA105010.13483408640629474No Hit
GTTTTGGTTAAAACTGTTAGTTTTGCCAGTTATATTTAAGTGCGTGTTTACGAGATCGCCTCTCGGCTCGCAGCT94050.12076131631760804No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAGCGCGGCGCAATTCA88630.11380197198542905No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGATTAA79980.10269526931506955No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACT79250.10175794065040338No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTT79070.10152681851391035No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTT78750.10111593471570052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG38300.046.8392150
TTAGGGG28400.038.38558649
TTTAGGG32300.030.0131548
ATAGGGG15550.027.51000849
GGGGAAT48750.027.24666863
AGGGGGG93550.026.18351251
TATAGGG15000.024.37936448
ATTCGAC73750.023.7672655
GATTCGA74750.023.5899094
GGCGTGA32700.022.3797551
GGGGTAA26600.021.79044566
TCGACAG80950.021.6946837
TTGGGGG71350.021.2263650
GGATTCG86850.020.6618713
GTGATAG36950.020.6392734
GGGGAAA125900.020.49681922
CGGATTC90250.020.3829152
CGACAGG86500.020.301938
ACTAGAT99800.020.04983552
TAAGGGG18950.020.02573650
Produced by FastQC (version 0.11.2)