FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_79t2_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_79t2_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5599462
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA87920.15701508466349087No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG61720.11022487517550793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG21900.034.1841150
TTAGGGG19300.029.6723349
TTGGGGG44100.025.50278950
TTTTGGG40550.025.09871148
TTTGGGG41600.024.3811949
GGTTGGT10000.023.11320531
GATTCGA30650.022.9666444
AGGGGGG39200.022.88210351
GAGGGTT6750.022.48593727
GGGGAAT30000.022.3106663
GGGGAAA53400.022.15898522
CTAATTA7250.021.895193
ATTCGAC34400.020.7626425
GTTTGGT16300.019.8941631
TGGGGGG61700.019.7936951
GTGATAG19900.019.7674054
GGGGGAA60950.019.41410821
GGATTCG37350.019.4024963
TTTAGGG30850.018.89956748
TACAAGG37750.018.7356538
Produced by FastQC (version 0.11.2)