FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_79t1_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_79t1_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7745547
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG47000.048.9581150
TTAGGGG35850.044.55389849
TTTAGGG44900.035.19108248
GGGGAAT59100.034.55815563
AGGGGGG85200.032.9599951
GAGGGTT18100.032.78050627
GGGGTTA37350.032.4214865
GGTTGGT34800.031.32434831
GGGAGAG38850.030.72527523
GAGAGGG26000.030.38379125
GGGAGTT15900.030.16018164
GGGGAGA63900.030.07218622
GGGGTAA46550.029.64554466
GGGGGTA51950.029.41944565
GAGGGGT30900.028.02083427
GTTTGGT47750.026.94695331
TTGGGGG174250.026.66806450
GGAGAGG31050.026.66477424
TTAAAAA150150.026.26392669
TTTTGGG142600.026.12869348
Produced by FastQC (version 0.11.2)