FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_79t0_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_79t0_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6297676
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG7400.024.24183750
AGGGGGG12400.017.80556151
TTAGGGG8700.016.65407449
CTACCGA4051.4551915E-1114.48156211
GGGGGGA24800.014.18925720
TTGGGGG18600.013.53953750
GACTAGT1550.00811762913.354382523
CGGGGAG8900.013.1867611
CTAACGT15800.013.1082091
GCCTAGC13250.012.23751260
CTAGACT2000.003101819712.07439451
GGGGGAA25600.011.99401762
CTGGGTT12300.011.7867321
TCCCCTA7750.011.57444164
CCCTAAG7200.011.49960622
CGGGAAT24450.011.4355061
TACTGCG8750.011.43434918
TTTGGGG21600.011.17981749
CCCGGGT7450.011.1199851
CCTACCG5301.3587851E-911.06627510
Produced by FastQC (version 0.11.2)