FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_79t0_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_79t0_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7584420
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297920.39280525076406636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG164350.057.3047250
TTAGGGG119500.057.10184549
TTTAGGG120600.055.23693548
TATAGGG26050.052.9720648
GAGAGGG53700.051.2608725
GGGAGAG89800.047.7155523
TTTTAGG133600.046.0662747
ATAGGGG32100.045.88963349
TTAAAAA287500.045.48433769
GGAGAGG64300.045.11752724
AGAGGGT30600.043.2873926
TTATAGG25950.043.0727247
TGGGGGA127800.041.81254233
GGAGAGT29550.041.44108224
AGGGTTG48300.040.92248528
GGGATTA66800.040.5963165
GGGGAAT181550.040.1931263
AGTGTGG32400.039.92461828
GGGATGT56450.039.78376436
GGGGTTA114050.039.568865
Produced by FastQC (version 0.11.2)