FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_79t-1_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_79t-1_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5547733
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG21350.048.63782550
TTAGGGG19250.043.1900849
TATAGGG5350.038.04588348
GGGGAAT32800.034.6062963
TTTAGGG26400.033.453848
AAGGGGG17100.028.4464851
ATAGGGG7150.027.98463649
TTGGGGG40750.027.76845750
TAAGGGG13500.027.59913850
TTTTGGG41650.026.9201748
AGGGGGG39250.026.72018451
TTTGGGG41300.026.31226349
GGGGAAA72350.026.08257122
GGGAATA23400.023.73750964
TGGGGGA12800.023.7180120
TAGGGTG3250.023.35311750
GAGAGGG7200.022.99803725
GGGGAGA19100.022.93886822
TAGGTGG3950.021.83476350
GGGGGAA87300.021.3007962
Produced by FastQC (version 0.11.2)