FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_659t0_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_659t0_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9781353
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG22300.041.61531450
TTAGGGG22600.031.59810349
AGGGGGG42900.022.83869451
TTGGGGG43850.022.5010250
ATAGGGG9850.022.41521349
TTTAGGG33000.022.37226948
TATAGGG9050.021.34763148
GGGGAAT44100.020.57535663
TTTGGGG49200.019.63324249
TTTTGGG53350.018.7531348
AAGGGGG24450.018.62538551
GGGGGGA113000.018.19655220
GGGGAGA27900.017.8062722
GGGGAAA87000.017.8049922
GGGGGAA92350.017.63300762
TAAGGGG19450.017.55987450
GGGAATA31600.015.17586564
GGGGGTA18400.015.00046165
GGGGGAT22450.014.44580463
CGGGGAG22400.014.1782711
Produced by FastQC (version 0.11.2)