FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n02_659t-1_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n02_659t-1_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5416914
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG23500.052.84852650
TTAGGGG21950.048.5639749
TTTAGGG28500.037.16171348
TTGGGGG53050.033.9455950
GGGGAAT35300.032.4481363
AGGGGGG40000.032.4287451
TATAGGG5100.031.79288548
TTTGGGG54050.031.46599249
TTTTGGG57150.030.78620548
GGGGAGA24200.027.08597822
GAGAGGG8950.025.8248625
ATAGGGG6550.025.80744649
GGGGGTA19850.025.20216265
TAAGGGG11450.024.70623650
GGGGAAA67300.024.29794522
GGGGTAA19700.024.16813766
AAGGGGG17750.023.51739751
TGGGGGG78100.023.10217551
TGGGGGA10750.021.82281120
GGGGTTA16650.021.75731865
Produced by FastQC (version 0.11.2)