FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_wt0_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_wt0_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11305136
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1225331.083870198465547TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG115940.1025551572311912TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCTC172800.059.156740
GCTATCT174000.058.72889739
TATCTCG174000.058.6892441
GTATGCC186650.055.37705647
GTCACCT187750.055.1261129
AGTCACC187900.055.0270228
GAACTCC188600.054.84107621
GAAGCTA188150.054.77054236
TCGTATG189700.054.46851745
CTCGTAT188500.054.43091644
TATGCCG194500.053.1775248
AAGCTAT194800.052.9008137
TCCAGTC196450.052.8252825
CTCCAGT198200.052.35886424
CAGTCAC197650.052.34746627
AGCTATC196000.052.20728338
CCAGTCA199150.052.0224826
ACACGTC200900.051.82691213
ACTCCAG201450.051.49703623
CACGTCT202750.051.2178914
Produced by FastQC (version 0.11.2)