FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_wt-1_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_wt-1_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487068
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA617020.9511538957199153TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG109200.058.2262741
ACTCCGC133050.057.9015432
GTATGCC112750.057.55616447
CTCCGCG134200.057.19970333
CACTCCG137050.056.11091631
GTCACTC137700.055.79593329
TCGTATG110450.055.75606545
AGTCACT140200.054.97325528
TCACTCC140150.054.82055330
CAGTCAC140950.054.63178627
GAACTCC141300.054.42321421
CGTATGC119050.053.75689346
CCAGTCA143000.053.752126
TATGCCG120950.053.62552648
CTCGTAT114700.053.5096644
TCCAGTC144900.053.07108725
CTCCAGT147100.052.27736724
CGCGAAA147150.052.18926236
GCCGTCT110950.051.61750851
TGAACTC149150.051.55883820
Produced by FastQC (version 0.11.2)