FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_vgt2_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_vgt2_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6195457
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA645731.0422637103283905TruSeq Adapter, Index 7 (97% over 35bp)
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA97520.157405660308836No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG62690.10118704721863132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG86000.061.45766428
GTATGCC88050.059.0477247
ACTCCAG89950.058.60544223
TCGTATG89100.058.08082645
TATCTCG89450.057.81453341
CGTATGC90400.057.43641346
ATTCGTA92850.056.8864836
GTATCTC91400.056.6943140
GCACACG95700.055.5528611
GTCACGA95950.055.44407329
CACACGT96150.055.1134612
CGTATCT94500.054.90751339
CTCGTAT94800.054.51584244
CTCCAGT98350.053.7403124
TGAACTC99250.053.7396320
ACGTCTG100100.052.93865615
TCCAGTC99850.052.89843825
TTCGTAT100150.052.67108537
CAGTCAC100850.052.64758327
AGCACAC101050.052.64580510
Produced by FastQC (version 0.11.2)