FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_vgt1_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_vgt1_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7035611
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA345250.490717863736355TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC57700.051.24146347
AGTCACA60150.050.0720228
AATCTCG57600.049.29397241
CGTATGC61400.047.81648646
TCGTATG61250.047.31400345
ACTCCAG64050.046.96927323
GAACTCC65250.046.4755921
CTCCAGT65450.045.96458424
TGAACTC67500.044.77306720
TCCAGTC68800.043.6763425
GTCACAT69000.043.39974229
CTCGTAT66050.042.93540244
TATGCCG68750.042.90519748
ACACGTC72400.042.21936413
CACGTCT72150.041.935314
GCCGTCT70250.041.05598451
CACATTC73450.041.00520331
AACTCCA74550.040.77038222
ACGTCTG76400.039.69282515
CCAGTCA76650.039.38332726
Produced by FastQC (version 0.11.2)