FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_vgt0_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_vgt0_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7496823
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA414650.5531009602334216TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG59200.054.95490341
AGTCACG65000.051.32507328
GAACTCC66250.050.35667421
GTATGCC67750.049.0380847
TCGTATG71450.046.5952445
TGAACTC71700.046.43277420
GTCACGA72050.046.3987529
CGAGATT72300.046.1905933
CTCGTAT72850.045.46300544
CGTATGC73850.045.12768646
TCCAGTC74950.044.55743425
CCATCTC73900.044.25683240
ACTCCAG76350.043.96633523
CTCCAGT76250.043.8882624
CACGTCT76350.043.83077614
ACGAGAT77000.043.3263632
GCCGTCT76850.043.2762551
ACACGTC77500.043.13586413
TATGCCG77900.042.7815148
CACGAGA79600.042.60463731
Produced by FastQC (version 0.11.2)