FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_sigg45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_sigg45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2886866
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA29370.10173662372967779TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGC10050.035.70120633
TCTCGCG10250.035.3411834
CTCGCGC11250.031.58641235
TCGCGCA11750.030.53592536
GAACTCC12050.030.06221
CGCGCAT12000.029.6122637
ACTCCAG12850.028.99587423
CATCTCG10750.027.5997841
ACACGTC13600.027.3968413
CACTCTC13900.026.5573431
GTATGCC11700.026.53824847
CTCCAGT14000.025.87479424
TCCAGTC14400.025.87479425
TCGTATG11350.025.53283545
GATTAGT7350.025.3467359
CGATTAG7600.024.5129628
TGAACTC14900.024.31188620
AGTCACT15450.023.89301328
ACTCTCG15500.023.81593732
CGTATGC12900.023.53469546
Produced by FastQC (version 0.11.2)