FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_sige45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_sige45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7007387
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCTCAGACCTATCAACCTGATCATCTTT172340.2459404625433132No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCC131180.1872024479310191No Hit
GCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGAGACT118600.16924996435903997No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGGGTTTCCCCATTCGGAAAT114310.1631278535065924No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTC109390.1561066914100791No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAG107450.15333818440454336No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA107020.15272454625383183TruSeq Adapter, Index 7 (97% over 36bp)
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAA104380.1489570934215564No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTAAGCCCGACTTTCGTCCCTGCTCGAC96820.1381684784927677No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCG89070.1271087211252925No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACT88680.1265521655932518No Hit
CGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAG86340.1232128324010077No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCG85740.12235659312094507No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCTGCTTCTAA85040.12135764729420538No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTACCGCT81850.11680530845520593No Hit
CACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCA78450.11195328586818454No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTCGGTGGAGGGGATTCTCACCCCTCTT74190.1058739869797401No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTA73430.10478941722499414No Hit
TTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTA72040.10280579622618247No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG71390.1018782036727813No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGGGTTTCCCCATTCGGAAATCTCCGGA70930.10122175355806666No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATGGGAGCTTGC70890.1011646709393958No Hit
CGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGGGCTGTAGTCGGTCCCAAGGGTTGGGCTGT70780.10100769373805099No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGAC70560.10069373933536138No Hit
CAAGAGTCCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGGGCTGTAGTCGGTCCCA70120.10006583052998214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC20550.044.65664747
TCGTATG20750.044.0599645
CCGGCTA20850.044.0141133
TCGATCG33100.043.1507843
CTATGAT21050.043.10423737
CTCGTAT21050.043.10423744
GGCTATG21150.043.06355335
GATTAGT33200.042.605159
CGATCGA33900.042.336014
TTAGTAT33450.042.18358611
CGTATGC21800.042.0960646
CGATTAG34150.041.5209668
ACTCCAG22100.041.3685123
GCTATGA22050.041.30585536
ATTAGTA34450.041.05924610
GAACTCC22750.040.48985321
GATCGAT35550.040.2740025
AGTATCT35200.040.18440213
TCCAGTC23500.039.1976225
ACACGTC23550.039.114413
Produced by FastQC (version 0.11.2)