FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_sige35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_sige35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5887241
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG363680.6177426743698788No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA336530.5716259959461486No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC331990.5639144040476685No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAG198000.3363205277310713No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGATTAA176030.2990025378611135No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCT173610.29489195363328935No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA158200.2687167044800782TruSeq Adapter, Index 8 (97% over 36bp)
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA155660.26440228962938667No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTT148950.2530047606340559No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGA148940.25298777474881695No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAGCGCGGCGCAATTCA129210.21947462317238245No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACT123890.21043813222526475No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTT121370.20615768914505114No Hit
CGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAAC118060.20053536113096102No Hit
GGCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGA114590.1946412589530478No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTT112730.19148188429860438No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAGCGCGGCGCAATTCAA109480.18596147159594792No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTA104620.1777063313698216No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGCAGCTGGCGGCCATTAATCTCATAT100800.1712177232085454No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAAC97140.16500088921109227No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAATGAAAAAAGCTTATAAGAAACCTCGC91310.15509811811678847No Hit
GAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGAT86060.1461805283663434No Hit
CGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAGT85100.14454988338340488No Hit
GTTTTGGTTAAAACTGTTAGTTTTGCCAGTTATATTTAAGTGCGTGTTTACGAGATCGCCTCTCGGCTCGCAGCT80740.13714403741922576No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAAC77260.13123294935607357No Hit
GTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAGTC77050.13087624576605578No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGG72340.12287589381851363No Hit
TATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTG72020.12233234549086744No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGCAGCTGGCGGCCAT71520.12148305122892031No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAGCGCGGCGCAATTCAAAAAGGCGAGG62920.10687518992342933No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACC62590.10631465571054421No Hit
CTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAGTCTG59660.1013377913355339No Hit
CTATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGT59150.10047151118834782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCG35800.054.7370932
TCTCGCG36950.052.84676734
TCACTCT37750.051.81822230
TCGCGCA37950.051.45422736
CGCGCAT37850.051.2255837
CACACGT38550.051.0113612
CTCTCGC38750.050.92613633
CATCTCG32650.050.50811441
GCACACG39100.050.38204611
CTCGCGC38850.050.35104435
ACTCCAG39300.049.8622923
GTATGCC34750.048.15078447
GAACTCC41200.047.64655321
GTCACTC41450.047.27594429
CCAGTCA41700.046.99251626
TCCAGTC41850.046.7416525
ACACGTC42700.046.69994413
AACTCCA42200.046.68099222
AGTCACT42100.046.6279828
CAGTCAC42800.045.9459827
Produced by FastQC (version 0.11.2)