FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_s877_sigg45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_s877_sigg45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7783787
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA171910.22085650596554096TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT30150.047.0295839
ATAGATC33150.043.08572438
GCGATAG33400.042.96980735
GATAGAT34500.041.29976337
AGATCTC34450.041.25955640
AGTCACA36700.039.5760728
ACTCCAG37050.038.8297423
CGATAGA37600.038.07823636
GAACTCC39700.036.4116321
GTCACAG39750.036.1922429
TCGTATG40300.035.95512845
GTATGCC40500.035.69238747
CTCGTAT40250.035.65693744
ACACGTC42200.034.499813
CTCCAGT42000.034.4176624
CACAGCG42400.034.255731
AGCGATA42950.033.57605734
CGTATGC43350.033.50499746
TCCAGTC44300.032.7864925
AGCACAC45200.032.36264410
Produced by FastQC (version 0.11.2)