FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_s87745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_s87745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8429084
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA111240.13197163535207385TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG35350.038.06192833
GAACTCC39450.034.19364521
ACTCCAG41050.032.94492723
TCCAGTC41500.032.42142525
GATTAGT14650.032.262549
ACACGTC42500.032.226813
AATCTCG34800.032.02129741
CTCCAGT43850.030.68390524
GTATGCC38150.029.93309647
CACGTCT46650.029.06407714
CGCGAAA46650.028.84221536
CCGCGAA46800.028.74977135
CGTATGC38450.028.35364746
TCGTATG38150.028.21488245
CCTAACT10050.028.149532
ACGTCTG49050.027.57164415
CTCGTAT39700.027.28709844
AGCACAC50000.027.11678310
CACTCCG50200.027.07747531
ACTCCGC51400.026.78091432
Produced by FastQC (version 0.11.2)