FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_csfg_sige35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_csfg_sige35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6782548
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG275000.405452346227406No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC225820.3329427230002648No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA221450.3264997166256693No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA207030.3052392699616722TruSeq Adapter, Index 1 (97% over 36bp)
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAG130230.1920074874516185No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGATTAA115130.16974446771331364No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGA111100.163802747875872No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA107300.15820013363709332No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCT101500.1496487750621153No Hit
GGCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGA98870.14577117626001318No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTT86500.12753319254062043No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAGCGCGGCGCAATTCA84570.1246876542561881No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTT76920.11340870717022569No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACT76170.11230292804415096No Hit
CGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAAC74610.1100029074619155No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGCAGCTGGCGGCCATTAATCTCATAT72290.10658236403192428No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTT71810.10587466539123645No Hit
GTGAAATTGTAAATTAGAATTGTCCTCCGCCCATGTTTTGTTGAGCAAAAGATACAAGACGTTTTGTGATCTCTC68150.1004784632559917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT31650.051.88596339
GCGATAG33000.050.2860835
GATAGAT34950.047.87526337
ATAGATC36350.045.46191838
CGATAGA37400.044.9235436
ACTCCAG37550.044.37658323
ACACGTC38550.043.76239413
TCCAGTC38800.042.9469225
GAACTCC38950.042.78152521
CTCGTAT39650.041.5911944
AGCGATA40250.041.48547734
AGATCTC39600.041.46946340
TCGTATG41400.040.33310745
CACACGT41800.040.27728312
GTCACAG42350.039.83566729
GTATGCC42050.039.62759847
AGTCACA45150.038.5878228
GCACACG43700.038.2892611
AACTCCA44250.037.96927622
CGTATGC44500.037.6009146
Produced by FastQC (version 0.11.2)