FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_csfg35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_csfg35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8297743
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA250630.3020459900963431No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA200340.24143914797071925TruSeq Adapter, Index 6 (97% over 36bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG172180.20750220873314587No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC164270.19796949604247807No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAG123700.14907668265936894No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA109360.13179487482318988No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTT94070.11336817734653869No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACT91240.10995761136492176No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGATTAA87500.10545036162243156No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCT86940.104775479308048No Hit
CGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAAC85680.10325699410068497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG46750.050.03391633
GTCACTC49100.047.49868829
CACTCCG49650.046.97252331
CACACGT53100.044.83023512
ACTCCAG52550.044.77422723
ACACGTC53650.044.24204313
AGTCACT53850.043.4370628
ACTCCGC55650.042.71402432
GAACTCC55500.042.20784821
CTCCAGT55750.042.1423424
CAGTCAC57150.041.23071727
AACTCCA57450.040.77520822
CCGCGAA57550.040.76430535
TCCAGTC58500.040.51513725
CCAGTCA58850.040.21555726
TCACTCC58550.040.00914830
AATCTCG50700.039.26307341
GCACACG60900.038.86183511
ACGTCTG61600.038.42022315
GTATGCC51850.038.25916747
Produced by FastQC (version 0.11.2)