FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_79t45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_79t45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7048888
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA20900.035.8203629
CACTAAT21500.034.98118631
ACTAATG24500.030.41614532
CATCTCG23900.026.70486341
TCACTAA29400.025.81617230
AGTCACT29000.025.81536328
ATGCGCA29100.025.72665236
ACTCCAG29550.025.33487523
TAATGCG30950.024.85768934
TGCGCAT30250.024.63456337
CAGTCAC30250.024.63456327
GAACTCC31050.024.22207621
CACACGT31500.023.65700112
CTCCAGT33450.022.69044924
ACACGTC34000.022.52633713
CCAGTCA33850.022.21848126
GTATGCC30000.022.0798747
AACTCCA35350.021.7636622
CTCGTAT29050.021.37680844
GCACACG35850.021.17142311
Produced by FastQC (version 0.11.2)