HVKTVAFXX_n01_79t35.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HVKTVAFXX_n01_79t35.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7788090
Sequences flagged as poor quality	0
Sequence length	75
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	81151	1.0419884721414365	TruSeq Adapter, Index 3 (97% over 36bp)
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA	22382	0.2873875366103884	No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG	21184	0.2720050744149079	No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC	19201	0.24654311904459245	No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTTCCGAGAGCTAG	10845	0.13925108723705043	No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA	10612	0.1362593395813351	No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCT	9995	0.12833698634710178	No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGATTAA	9775	0.1255121602344092	No Hit
GTTTTGGTTAAAACTGTTAGTTTTGCCAGTTATATTTAAGTGCGTGTTTACGAGATCGCCTCTCGGCTCGCAGCT	9239	0.11862985661439454	No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAGA	8468	0.10873012510127643	No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCCTGCGGGCGGGCCTT	8123	0.10430028415182671	No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTT	8093	0.10391508059100499	No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACT	8079	0.1037353189292882	No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTA	7881	0.10119297542786486	No Hit
CGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAAC	7790	0.10002452462670565	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTCACTA	15060	61.852123	29
CACTAAT	15225	61.023186	31
ACTAATG	15350	60.661106	32
CATCTCG	13390	59.131363	41
TAATGCG	15780	58.986248	34
TCACTAA	16060	58.065254	30
TGCGCAT	15975	58.007072	37
AGTCACT	16115	57.845673	28
ATGCGCA	16155	57.424824	36
GTATGCC	14250	57.23325	47
ACTCCAG	16390	56.938255	23
CTCGTAT	13630	56.470226	44
TCCAGTC	16615	56.104904	25
CAGTCAC	16655	56.01159	27
TCGTATG	13575	55.911182	45
CGTATGC	14295	55.77398	46
GAACTCC	16745	55.772346	21
AACTCCA	16795	55.667934	22
GCGCATC	16670	55.09197	38
CTCCAGT	16980	55.061424	24

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content