FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_79t2_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_79t2_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5599462
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA483920.8642258845581949TruSeq Adapter, Index 2 (97% over 37bp)
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATAA96510.17235584418645933No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATGTCAAATGCTATATGAG72170.1288873823949515No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGC70200.12536918725406118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC68500.057.91942647
TCGTATG70800.055.6967645
ACTCCAG71750.055.0554823
TATCTCG71300.054.9674741
CGTATGC71950.054.9503946
ACCGCTC75050.052.5886832
AGTCACC76150.052.14616428
CTCGTAT76700.051.45737544
ATTATCT76250.051.35384839
CTCATTA77850.050.60861236
CAGTCAC80300.049.40822227
CACACGT80650.049.3221412
CCAGTCA80900.049.12707526
CTCCAGT82000.048.46805624
GCCGTCT81500.048.34209451
GCACACG82600.048.15775311
TCCAGTC82450.048.03614825
TTATCTC81850.047.96677840
ACGTCTG86350.046.0264115
TGAACTC86300.046.013120
Produced by FastQC (version 0.11.2)