FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_79t1_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_79t1_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7745547
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGG276800.061.96993332
ATCGGGG213300.061.7210842
CACTCCG279100.061.4839831
TCGGGGG90550.060.88416343
GTCACTC281550.060.83867329
AGTCACT281750.060.83222228
CGGGGGT77600.060.6413244
TCACTCC282600.060.6248430
GTGGGTG76200.060.3066659
CTCCGGA286400.059.9529633
GAACTCC287150.059.9525621
TCCGGAG285950.059.9387234
ACTCCAG288000.059.65582323
CTCCAGT288350.059.4398424
GCGGGGT97000.059.2542252
TCCAGTC290450.059.08135225
GAGAATC285250.059.05777438
TGAACTC292800.058.74855820
CCAGTCA292200.058.70389626
CAGTCAC294800.058.29147727
Produced by FastQC (version 0.11.2)