FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_79t0_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_79t0_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6297676
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA74220.11785299847118208TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCG26400.036.4600132
CTCTCGC26700.035.7919233
CATCTCG23000.032.69980641
CTCGCGC30200.032.10080335
TCTCGCG30500.031.8981734
CGCGCAT31800.030.05170837
TCGCGCA33600.028.95518536
GTCACTC32700.028.90808529
AGTCACT33750.028.11094328
TCACTCT34100.027.92358630
GTATGCC27950.027.7726447
GAACTCC34550.027.65974621
CACTCTC36100.026.85441231
CCAGTCA36400.026.15918426
GCATCTC29400.025.81617440
ACTCCAG38500.025.0011523
TCCAGTC38200.024.92655425
CAGTCAC39150.024.85042627
TGAACTC38400.024.79672820
GCGCATC38750.024.75081838
Produced by FastQC (version 0.11.2)