FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_79t0_vg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_79t0_vg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7584420
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7507279.898278312646188TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG507020.6685020080638995TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT288610.3805300866776893TruSeq Adapter, Index 27 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG148500.19579611888582119TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAT938150.065.5868737
TACTCGA948400.065.50371636
GTATGCC955550.065.2590847
TCGTATG955950.065.1848545
CGTATGC957650.065.15246
TCGATCT928900.065.081239
TTACTCG958950.064.876635
CTCGTAT944850.064.81142444
GCCGTCT961600.064.7049951
TATGCCG964000.064.6906348
ATTACTC963000.064.6467434
CTCGATC936750.064.6205238
ACATTAC968050.064.4199832
CATTACT970000.064.2371333
ATGCCGT970800.064.2161749
TGCCGTC972900.064.0988450
CACATTA977700.063.80885331
ATCTCGT952200.063.662642
GTCTTCT975700.063.66031354
TCTCGTA954300.063.6454243
Produced by FastQC (version 0.11.2)