FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_79t-1_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_79t-1_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5547733
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA486380.8767184722119828TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAT92150.060.46356231
GTCACTA94050.059.38880529
ACTAATG96150.058.01994332
CATCTCG83800.057.760441
TGCGCAT96400.057.54738237
ATGCGCA96850.057.3868636
GCATCTC84550.057.24803540
TCACTAA98000.056.99507530
GTATGCC86700.056.783447
GAACTCC101850.055.07773221
TCGTATG85100.055.05372645
AGTCACT102450.054.6204728
TAATGCG101900.054.57671434
CTCGTAT87400.053.8417944
CTAATGC103900.053.82499733
CGCATCT93050.053.46449339
CAGTCAC105000.053.42539627
CCAGTCA105300.053.10937526
GCGCATC104150.052.86767238
AATGCGC106000.052.53082735
Produced by FastQC (version 0.11.2)