FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_659t0_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_659t0_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9781353
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA604520.6180331085075859TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT89750.054.77683339
AGATCTC94500.052.02349540
GTATGCC100250.049.9343747
ATAGATC99550.049.4190938
GCGATAG103850.048.3362535
TCGTATG105000.048.03685445
CTCGTAT106550.046.65809244
GATAGAT108650.045.85154337
CGATAGA109600.045.73740836
GTCACAG111600.045.04139729
CGTATGC111450.045.0091546
AGTCACA111400.044.93644728
GAACTCC113500.044.1354221
TATGCCG116700.042.92519448
GCCGTCT117900.042.63460551
CAGTCAC118800.042.25353227
CCAGTCA119700.041.84937726
CACAGCG120150.041.8074931
CTCCAGT121100.041.56498324
TCCAGTC121600.041.4791925
Produced by FastQC (version 0.11.2)