FastQCFastQC Report
Mon 11 Dec 2017
HVKTVAFXX_n01_659t-1_w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKTVAFXX_n01_659t-1_w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5416914
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA736061.3588179542817185TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG65370.12067756659972818TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC109100.057.83701747
CCGGCTA112700.055.8364633
TCGTATG112850.055.73166745
GCTATGA113050.055.5415236
CTCGTAT112850.055.1813844
TATGCCG115050.054.7559248
CTATGAT114300.054.5115437
AGTCACC115550.054.39955528
GGCTATG116000.054.21826635
TCACCGG118100.053.2833930
GCCGTCT118250.053.1866351
CGGCTAT119400.052.70325534
CAGTCAC121800.052.0046727
CGTATGC122850.051.67251646
ACCGGCT122350.051.658132
CACCGGC122000.051.55179631
CCAGTCA122850.051.25127426
GAACTCC124250.050.64602721
ATGCCGT125000.050.6181549
TGATCTC122300.050.40980540
Produced by FastQC (version 0.11.2)