FastQCFastQC Report
Thu 23 May 2024
HVKNTDMXY_n02_CITE-seq_test2_BCells_SC_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKNTDMXY_n02_CITE-seq_test2_BCells_SC_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18026
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3051.692000443803395No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1450.8043936536114502No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC1300.7211805170309553No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1130.6268722955730611No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC770.4271607677798735No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC690.3827804282702763No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT570.3162099190058804No Hit
GGGAGAAGTAGAGGGATCCAGGCCATGGTGAGACACCCAGAGTTCTGCCT470.2607344946188838No Hit
CCTCAGAGCCTGTGCTGAGAGTGAGGAGGGGGAGAAGTAGAGGGATCCAG430.2385443248640852No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT390.2163541551092866No Hit
CAGACACCGAGGGTGGCTGTGTCAACTCAGAGGAGGCCTCAGAGCCTGTG370.20525907023188728No Hit
GCTGTGTCAACTCAGAGGAGGCCTCAGAGCCTGTGCTGAGAGTGAGGAGG370.20525907023188728No Hit
GTAGAGGGATCCAGGCCATGGTGAGACACCCAGAGTTCTGCCTCCTGGAC350.19416398535448795No Hit
GTCCTGGGGACACAGACACCGAGGGTGGCTGTGTCAACTCAGAGGAGGCC340.1886164429157883No Hit
GTCAACTCAGAGGAGGCCTCAGAGCCTGTGCTGAGAGTGAGGAGGGGGAG320.177521358038389No Hit
GAGGGATCCAGGCCATGGTGAGACACCCAGAGTTCTGCCTCCTGGACCCA300.16642627316098968No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG300.16642627316098968No Hit
CACAGACACCGAGGGTGGCTGTGTCAACTCAGAGGAGGCCTCAGAGCCTG290.16087873072229003No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG280.15533118828359038No Hit
CTCAGAGCCTGTGCTGAGAGTGAGGAGGGGGAGAAGTAGAGGGATCCAGG270.1497836458448907No Hit
GCCTGTGCTGAGAGTGAGGAGGGGGAGAAGTAGAGGGATCCAGGCCATGG270.1497836458448907No Hit
GGGGGAGAAGTAGAGGGATCCAGGCCATGGTGAGACACCCAGAGTTCTGC260.14423610340619106No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG240.13314101852879176No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT230.12759347609009208No Hit
CATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGACACAGACACC220.12204593365139242No Hit
ACTCAGAGGAGGCCTCAGAGCCTGTGCTGAGAGTGAGGAGGGGGAGAAGT220.12204593365139242No Hit
CTCCAGGTCCAGTCCATGGTGTGGAGCTGTGGTCGAGGGGCTTCTTCTTA210.11649839121269277No Hit
GTGAGGAGGGGGAGAAGTAGAGGGATCCAGGCCATGGTGAGACACCCAGA210.11649839121269277No Hit
CACCGAGGGTGGCTGTGTCAACTCAGAGGAGGCCTCAGAGCCTGTGCTGA210.11649839121269277No Hit
GGTGATCCTGGCCGTCTGTCCTGGGGACACAGACACCGAGGGTGGCTGTG200.11095084877399312No Hit
GCCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCTTCCAGG200.11095084877399312Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGAAGTAGAGGGATCCAGGCCATGGTGAGACACCCAGAGTTCTGCCTC200.11095084877399312No Hit
CTCTCAGTGTCCTTATAAATCGCCGCCACAGGGGCCTGGCCCGGCTTTTG200.11095084877399312No Hit
CTGAGAGTGAGGAGGGGGAGAAGTAGAGGGATCCAGGCCATGGTGAGACA200.11095084877399312No Hit
CTCAGAGGAGGCCTCAGAGCCTGTGCTGAGAGTGAGGAGGGGGAGAAGTA190.10540330633529346No Hit
GGCCTCAGAGCCTGTGCTGAGAGTGAGGAGGGGGAGAAGTAGAGGGATCC190.10540330633529346No Hit
CGGCCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCTTCCA190.10540330633529346Illumina Single End PCR Primer 1 (96% over 33bp)
GGTACCAATAAGCATACTGCTTTGGCAATGCATCTCCAGAGCAGGTGATC190.10540330633529346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)