FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_h9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_h9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9898812
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122060.1233077262200757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA47550.019.95301469
TGTGTAG64050.019.12209769
CTGTGTA76050.016.55946768
GTATAAA20900.014.0258721
CTCTGTG123650.013.839293569
TAGGCCG8450.013.4680135
TCTACTC281450.013.2755869
ATCTACT354150.013.18065269
TCTGTGT106850.013.076961569
CTACTCT228850.012.52784669
TACTCTG191650.012.51129669
GATCTAC430700.012.48813369
TAAGGGT12150.011.6367554
GTATTAA32250.011.2283731
TAACCCG6201.2732926E-1111.1246045
GTCTAGG15050.010.9992221
CGATCTA492100.010.99373868
GTATAGG13500.010.9847941
ATCTAGG21350.010.8226631
AATACCG27300.010.7374845
Produced by FastQC (version 0.11.2)