FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_h8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_h8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6104863
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63530.10406457933617838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTG66300.013.8938969
GTATAAT18000.013.4135841
TATACTG15150.013.2036965
GTATTAA27900.012.9808881
GTATATA17300.012.7600741
TATACAG14550.012.32595355
CTACTCT80150.012.22477669
TATTATA30050.011.9353032
TCTACTC93900.011.904345569
ATCTACT122000.011.84896269
GTCTAGG10250.011.77778151
GTGTAGA26300.011.54391269
TATAATA29450.011.4758632
CGATCTA158550.011.445785569
GTATTAT29050.011.2796791
TGTGTAG28550.011.2383669
GATCTAC143800.011.22827669
TAATACT29650.011.1667484
ATCTAGG13300.011.1515731
TATACGG7150.011.0943525
Produced by FastQC (version 0.11.2)