FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_h7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_h7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5655845
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66020.11672880002899656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTAG38000.021.24530469
GTGTAGA30950.019.8422169
TAGACAG8000.019.8331155
TATACCG5600.016.630255
CTCTGTG85800.016.52666569
GTATAGA9400.016.5124511
TTAGACA10950.016.379654
TACTCTG162700.016.34923769
TATACAG13200.016.2009645
TCTGTGT67500.015.94709269
CTGTGTA53400.015.31274868
ATCTACT328650.015.26375769
CTACTCT199450.015.1011869
TCTACTC249850.014.74764269
GTCTAGG6900.013.4971341
GTATAAA21100.013.4047371
GATCTAC404300.013.22691769
GAGTCGC10200.013.1882611
CTAGAGG11150.012.9920683
GTCTAGT13150.012.8527851
Produced by FastQC (version 0.11.2)