FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_h6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_h6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6573863
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA36000.021.17931269
TGTGTAG54250.018.88768669
CTGTGTA69200.014.90768868
AATACCG8200.013.8800135
GATCTAC279500.013.25697869
ATCTACT230300.012.94320669
TATACCG4853.1286618E-1012.0891855
CGATCTA324150.011.56987868
TCTACTC188750.011.40564169
ATACCGC5906.002665E-1111.1080476
TAAGGGT10200.010.8199794
TACTCTG139750.010.7635869
CTACTCT159650.010.7617469
GTATATA23400.010.7607651
TAATACT20850.010.7518674
GTCTAAT13950.010.6323771
ATACCGT9800.010.5592066
TTAATAC20000.010.51888753
TATAATA22300.010.5164082
GTATTAG17150.010.4586421
Produced by FastQC (version 0.11.2)