FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5894052
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT29700.014.2832231
TCTACTC82250.014.00920569
GTATAGA9500.013.4324651
GTATAGG10600.013.3399921
GTATAAA15150.012.9759691
CTACTCT67700.012.79051569
GTATTAA25100.012.6412981
GTATAAT18900.012.5911351
GATCTAC131200.012.46371469
TACTCTG58800.012.20362969
TAAGGGT8550.012.1011144
GTATTAG19150.012.0665651
ATCTACT105100.011.88252769
TATAGAC16950.011.8005632
CGATCTA140250.011.68505168
TTAGGGT9750.011.6729214
TATAATA26400.011.6260042
TTAGACA13150.011.539774
TATTATA28050.011.4339022
TATACAG15000.011.2667125
Produced by FastQC (version 0.11.2)