FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_h1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_h1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4693162
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA24550.014.89624369
TGTGTAG27800.014.64399669
CTACTCT97600.014.24549869
GTATAGG6600.014.1080261
GTATTAT23250.014.0911691
TCTACTC119700.013.63293669
TACTCTG82350.013.61576869
GTATTAG15400.013.43621351
GTATAAG9250.013.4216891
GTATAGA8000.013.3634351
TATACGG6200.013.3493875
GTATTAA23150.013.2582251
TATAGTG10450.013.2003515
TAGGCCG3204.2081047E-712.93221955
CTGTGTA34800.012.78889169
TATTATA22700.012.6087162
TATAATA19900.012.4766512
ATCTACT151800.012.40918569
GATCTAC185000.012.27090569
TATAAGG8350.011.9765072
Produced by FastQC (version 0.11.2)