FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_g9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_g9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12766305
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAG108750.015.45026269
GGATAGT91400.013.8156469
TATACAG33250.013.3803995
GTATTAT59750.012.6999421
GTATATA39750.012.321631
GTATTAG45750.012.2889141
TATACCG18150.012.1611415
TACTATA48450.011.9577862
GTATTAA61850.011.9341371
GAGGATA151550.011.88367469
GTCTAGG20450.011.8065151
CCTATAC23700.011.7870733
GTATAGA26850.011.5615261
TATAATA65950.011.4515582
TATTATA63650.011.4319242
AGAGGAT195350.011.42686369
AATACCG42250.011.3464265
GTATAAA40600.011.2990661
TATAGTG29750.011.1289775
GTATTAC54600.011.0550921
Produced by FastQC (version 0.11.2)