FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_g6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_g6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10528617
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAG103900.014.97541569
AGAGGAT191650.014.00517869
TATACAG27650.013.9701955
GTATAAT33200.013.2985741
GGATAGT85250.013.27386369
GAGGATA146050.013.06295669
GTATAGA19650.012.81426051
TATTATA50900.012.7378742
GTATTAA53600.012.5488241
GTATATG21500.012.5138051
GTATTAT51600.012.2998941
TATAATA55350.012.0253172
CGAAGAG341750.011.99294469
CTATACT22150.011.8336134
GTATAAA34750.011.7127921
AAGAGGA278700.011.698029569
TATACTG26750.011.6037475
GTATTAC48650.011.4150091
GAAGAGG320100.011.01496569
TATACCG15200.010.8912375
Produced by FastQC (version 0.11.2)