FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5039934
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56340.11178717816542835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT48550.014.92253669
GAGGATA84700.014.90768769
AGGATAG59400.014.63614569
TATAATA22350.013.5772152
AGAGGAT109700.012.89406469
GTATAGA10350.012.6633331
GTATAAT17600.012.5421511
AAGAGGA157350.011.75196969
GTATTAG15200.011.5726111
GAAGAGG183850.011.50295569
TTAGGGT9650.011.4353584
ATAGTGT38300.011.349769
ACCGCGC5552.4556357E-1011.18588
ATCTAGG10800.011.1776121
TAGTGTA30300.011.15824869
GTATATA14600.011.1032411
GTATTAT21450.011.0949811
TAATACA26600.011.0195644
ATATAAG13800.010.9971061
AGTGTAG23900.010.97054769
Produced by FastQC (version 0.11.2)