FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_g4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_g4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5531960
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAG68200.015.98633769
GTATATA13850.014.4448971
GTATAAT14700.013.8442971
GGATAGT54100.013.13760769
TATACTG14050.013.00939755
TTATACT12750.012.9839954
AGAGGAT118750.012.696794569
TATAATA24650.012.59201052
GATAGTG50000.012.558784569
GAGGATA91750.012.48470569
GTATTAA24200.012.4005271
AGTGTAG27050.012.37230969
TATTATA23850.012.2913612
TATAGCG3651.5508886E-712.2831045
GTATTAG17850.012.1741491
GTATTAC22150.011.6794881
TACACAG19750.011.524835
GTATTAT24750.011.2887551
CTATACT10750.011.2288924
TAGTGTA33200.011.11997468
Produced by FastQC (version 0.11.2)