FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6740180
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77280.11465569168775908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG37450.014.73899269
GTATAGA10200.014.2017651
GTGTAGA33550.013.98446869
AGGATAG88150.013.775969
GTATTAT29250.013.5601721
GTATTAA27850.013.1272541
GAGGATA119150.013.1160769
TAGTGTA45800.012.50473468
TATACTG15850.012.4026995
GTATTAC27200.012.2997431
TTAGACA13700.012.083464
ATAGTGT54300.011.94421669
ATCTAGG14650.011.7713321
GTCCTAA9700.011.7337111
AGAGGAT155250.011.73280669
CGAAGAG262550.011.66811269
GGATAGT74250.011.56917869
TATACCG8950.011.5602935
GTATAAT18700.011.4351861
GTATTAG20900.011.3866511
Produced by FastQC (version 0.11.2)