FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_g11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_g11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7236257
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89850.12416640260289263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA45600.024.05947369
TGTGTAG62750.019.51814369
CTGTGTA76750.016.09368568
CTCTGTG130500.015.30706469
TCTGTGT105450.014.72277869
CTACTCT255750.013.75968269
TACTCTG210300.013.69843869
GTATTAA22250.013.6410361
GTCCTAA7350.013.6083461
TCTACTC316800.013.53660769
ATCTACT396650.013.04690969
ATCCTAG20250.012.43347551
CCTAGAT19950.012.101633
TCCTAGA27100.012.0899642
GTATAGA11100.011.8074281
GTATAAT14250.011.6177241
GTATTAG16450.011.5316511
TATACAG13500.011.4968665
ACTCTGT203450.011.41251169
GATCTAC476200.011.30934269
Produced by FastQC (version 0.11.2)