FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n02_g10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n02_g10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234312
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30750.13762625810540338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA17400.030.93086469
TGTGTAG24300.023.42579769
TTAGGGT2800.019.7066734
GTCCTAA3400.019.272831
CTGTGTA33000.017.4609568
TCTGTGT45200.017.17357869
TAGAACA6700.016.9859754
TACTCTG108750.016.81370269
TATAGTG3700.016.7769285
TAGGATA2301.4307625E-716.4936284
TATACAG3401.4551915E-1116.228665
GCGCGTG1503.5319533E-416.09702711
TATACGG2151.2819401E-616.0399555
CGCGAAT5100.015.55629815
ATCTACT219250.015.38919669
TATAACG1808.119089E-515.3270682
TTAAGCA2051.4492638E-515.14049154
CGTTAAG1605.758383E-415.09468147
GTCTAGG3201.7062121E-915.0885961
CTCTGTG56700.015.02901769
Produced by FastQC (version 0.11.2)